The penetration of vitamin C with iontophoresis

      Relevance: The iontophoresis is a noninvasive method in which some ingredients, for example analgetic or anti-inflammatory substances, can be absorbed without pain. Today, a number of international researches are underway in connection with vitamin C iontophoresis. The use of iontophoresis with ascorbic acid by continuous electric current contributes to a quantitative gain of chondrocytes and improved the thickness distribution of calcified and non-calcified cartilage. Reproduction of collagen in the skin is increased by iontophoresis with ascorbic acid.
      Vitamin C plays an important role in the synthesis of collagen and thus it may contribute to regenerating articular surfaces. The anti-inflammatory effects of vitamin C may alleviate the symptoms of arthritis
      Purpose: To prove that under laboratory conditions the iontophoresis helps vitamin C transport through a gel matrix and pork skin tissue.
      To prepare a gelmatrix to be connected into an electrical circuit that can indicate the absorption of vitamin C during the iontophoresis.
      To determine the appropriate electricity current.
      To detect vitamin C in the solution with photospectrometer after the iontophoresis in the skin.
      Methods/analysis: The experiment consists of two test methods, intophoresis on the gelmatrix and through the skin tissue.
      The special gel matrix connected into an electrical circuit and contains an indicator that can turn to red if the vitamin C is being absorbed into this gel.
      On the gelmatrix, the iontophoresis was carried out from cathode with galvanic and diadynamic current (I = 10 mA, t = 20 min).
      The research has been modelled by the living tissue with pork skin and saline solution. The glass is filled with saline solution and then closed with pork skin, the iontophoresis was performed from cathode, with diadynamic current (I = 2 mA, t = 20 min).
      The amount of vitamin C in the saline solution after the iontophoresis can be determined with a photospectrometer.
      The control measurements have been carried out in every case when there was no flow of electricity.
      Three times each measurement were done, the results were averaged.
      Results: The results of penetration of iontophoresis on gelmatrix were on average 3.5 mm with diadynamic current and 3.3 mm with galvanic current, the control was 2.5 mm.
      In the skin, with diadynamic current the average result of vitamin C content is 11.01 mg/100 ml compared to the control group, in which it was 3.27 mg/100 ml.
      Discussion and conclusions: Compared to control the iontophoresis on the gel matrix and in the skin clearly demonstrated that the absorption of vitamin C is supported by electricity.
      The gel matrix can be connected in the electrical circuit and indicates the penetration of vitamin C with red color.
      Compared the two electric current there is no remarkable difference in the effectiveness of penetration.
      The photospectrometer measurement confirmed the presence of vitamin C in the saline solution.
      In the future the research will be continued with human trials.
      Impact and implications: The iontophoresis with vitamin C is non-expensive and easily accessible method for everyone.
      Funding acknowledgement: National Institute of Rheumatology and Physiotherapy, Budapest, Hungary. Semmelweis University, Budapest